Reactivity: | H,M,R & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | DDX21 rabbit polyclonal antibody |
Gene Name: | Nucleolar RNA helicase 2 |
Synonyms: | DEAD box protein 21, Gu-alpha, Nucleolar RNA helicase Gu, Nucleolar RNA helicase II, RH II/Gu, Ddx21, GURDB, GUA |
Immunogen: | Recombinant protein corresponding to Mouse DDX21 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. | 94 kDa |
Observed MW. | 95 kDa |
Uniprot ID: | Q9NR30, Q9JIK5, Q3B8Q1 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | testis |
IHC/IF | Mouse, Rat | 1: 500-1: 2000 | colon, testis |
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease.
Images
Western blot analysis of DDX21 (GB112112) at dilution of 1: 500 | |
Immunohistochemistry of paraffin embedded mouse colon using DDX21 (GB112112) at dilution of 1: 1000 (400x lens) | |
Immunohistochemistry of paraffin embedded mouse testis using DDX21 (GB112112) at dilution of 1: 1000 (360x lens) | |
Immunohistochemistry of paraffin embedded rat colon using DDX21 (GB112112) at dilution of 1: 1000 (400x lens) | |
Immunohistochemistry of paraffin embedded rat testis using DDX21 (GB112112) at dilution of 1: 1000 (300x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |