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| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Desmin |
Synonyms: | DES, CSM1, CSM2, LGMD2R, desmin |
Immunogen: | Recombinant protein corresponding to Mouse desmin |
Uniprot ID: | P31001 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm,Cell membrane |
Predicted MW. / Observed MW. | 54 kDa / 55 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 1000-1: 2000 | heart |
IHC/IF | Mouse, Rat | 1: 200-1: 1000 | heart+LPS, skeletal muscle |
Background
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures . May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction.
Images
|
Western blot analysis of Desmin (GB12081) at dilution of 1: 1000 |
 | Western blot analysis of Desmin (GB12081) at dilution of 1: 1000 |
 | Immunohistochemistry of paraffin embedded mouse heart+LPS using Desmin (GB12081) at dilution of 1: 500 (300x lens) |
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Immunohistochemistry of paraffin embedded rat heart+LPS using Desmin (GB12081) at dilution of 1: 500 (400x lens) |
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Immunohistochemistry of paraffin embedded rat skeletal muscle using Desmin (GB12081) at dilution of 1: 500 (200x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
