SuperLumin™ Dual Luciferase Assay Kit (100 T)

SuperLumin™ Dual Luciferase Assay Kit is a streamlined reagent system that facilitates rapid and straightforward quantification of stable luminescent signals from two reporter genes within a single sample. This user-friendly workflow is capable of generating both firefly and Renilla luciferase luminescence signals from cells that have not undergone preconditioning or pre-lysis, simplifying the assay process.

The Dual Luciferase Assay System allows for the establishment of internal controls, which are crucial for minimizing sample variability and reducing the incidence of false-positive and false-negative results due to nonspecific factors such as cytotoxicity. In this assay, the activity of the primary reporter gene is linked to the effects of specific stimuli, while the activity of the co-transfected control reporter gene serves as an internal control to normalize the results, ensuring accuracy and reliability.

Optimized for high-throughput processing of both 96- and 384-well plates, the system is compatible with a broad range of mammalian cell culture media, making it versatile for various experimental setups. The Dual Luciferase Assay Kit stands out as the premier choice for cell-based, high-throughput screening applications, offering a robust and efficient tool for dual reporter gene analysis.
 

Key Features

• Simplicity: Directly lyse cells in culture medium and measure luciferase activities without washing.

• Signal stability: The luminescent signal is highly stable with signal half-life of ~1 hr.

• Versatility: The assay is compatible with many eukaryotic cells (adherent or suspended).

• High-throughput: The assay system is adopted to high-throughput application.
 

Components

1. Dual Firefly Luciferase Substrate: 100 μl, 100×
2. Dual Firefly Luciferase Buffer: 10 ml, 1×
3. Dual Renilla Luciferase Substrate: 100 μl, 100×
4. Dual Renilla Luciferase Buffer: 10 ml, 1×
 

Storage

Store at 4°C.