Reactivity: | H,M,R |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Enoyl-CoA hydratase, mitochondrial |
Synonyms: | ECHS1, ECoH-1, Enoyl CoA hydratase 1, SCEH |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ECHS1 |
Uniprot ID: | Q8BH95 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 31 kDa / 27 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 1000-1: 3000 | testis, liver |
Background
Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate . Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate .
Images
Western blot analysis of ECHS1 (GB114604) at dilution of 1: 1000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |