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| Reactivity: | H & H |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | EGFR rabbit polyclonal |
Gene Name: | Epidermal growth factor receptor |
Synonyms: | EGFR, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, epidermal growth factor receptor |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human EGFR |
Isotype: | IgG |
Subcellular location: | Cytoplasm, Nucleus, Cell membrane, Secreted |
Predicted MW. | 134 kDa |
Observed MW. | 175 kDa |
Purity: | Affinity purification |
Uniprot ID: | P00533 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 500-1: 1000 | HeLa, A549, HepG2, A431, H1299, MCF7 |
IHC | Human | 1: 500-1: 1000 | esophageal cancer |
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
Images
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Western blot analysis of EGFR (GB111505) at dilution of 1: 1000 Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: A431 cell lysate Lane 5: H1299 cell lysate Lane 6: MCF7 cell lysate |
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Immunofluorescent analysis of paraformaldehyde-fixed human esophageal cancer using EGFR (GB111505) at dilution of 1: 800 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |