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| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Eukaryotic translation initiation factor 2 subunit 1 |
Synonyms: | EIF2S1, EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, eukaryotic translation initiation factor 2 subunit alpha |
Immunogen: | Recombinant protein corresponding to Mouse EIF2S1 |
Uniprot ID: | Q6ZWX6 |
Isotype: | IgG2a,κ |
Purity: | Affinity purification |
Subcellular location: | Cytoplasmic granule |
Predicted MW. / Observed MW. | 36 kDa / 38 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | colon, brain |
IHC/IF | Mouse, Rat | 1: 2000-1: 8000 | cerebellum, brain |
Background
Eukaryotic translation initiation factor 2 subunit 1 (eIF2α) is a protein that in humans is encoded by the EIF2S1 gene. The protein encoded by this gene is the alpha (α) subunit of the translation initiation factor eIF2 complex which catalyzes an early regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA (Met-tRNAiMet) to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (α, 36 kD, this article), beta (β, 38 kD), and gamma (γ, 52 kD). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2α.
Images
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Western blot analysis of EIF2A (GB12544) at dilution of 1: 1000 |
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Immunohistochemistry of paraffin embedded mouse cerebellum using EIF2A (GB12544) at dilution of (300x lens) |
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Immunohistochemistry of paraffin embedded rat brain using EIF2A (GB12544) at dilution of (300x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
