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Anti-EIF2S2/EIF2B Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | EIF2S2 rabbit polyclonal |
Gene Name: | Eukaryotic translation initiation factor 2 subunit 2 |
Synonyms: | eIF-2-beta, Eif2s2, Eukaryotic translation initiation factor 2 subunit beta |
Immunogen: | Recombinant protein corresponding to Mouse EIF2S2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 38 kDa |
Observed MW. | 50 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lung, colon, small intestine |
IHC | Human, Mouse, Rat | 1: 1000-1: 2000 | colon, lung, placenta |
IF | Human, Mouse, Rat | 1: 500-1: 3000 | colon, brain |
Background
Eukaryotic translation initiation factor 2 (eIF2) is composed of three subunits, eIF2 alpha, eIF2 beta (EIF2S2), and eIF2 gamma, which are present in equal molar amounts. eIF2 beta plays a central role in the maintenance of what is generally considered a rate-limiting step in mRNA translation. In the early steps of protein synthesis, eIF2 beta binds GTP and Met-tRNA and transfers Met-tRNA to the 40S ribosomal subunit. At the end of the initiation process, GTP bound to eIF2 beta is hydrolyzed to GDP and the eIF2/GDP complex is released from the ribosome. The exchange of GDP bound to eIF2 beta for GTP is a prerequisite to binding Met-tRNA and is mediated by eIF2 beta, which recycles the eIF2 complex for another round of initiation.
Images
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Western blot analysis of EIF2S2 (GB111135) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded human colon using EIF2S2 (GB111135) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded rat lung using EIF2S2 (GB111135) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat placenta using EIF2S2 (GB111135) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraffin-embedded human colon using EIF2S2 (GB111135) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraffin-embedded rat brain using EIF2S2 (GB111135) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
