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| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Eyes absent homolog 3 |
Synonyms: | EYA3, Eyes absent homolog 3 |
Immunogen: | Recombinant protein corresponding to Mouse EYA3 |
Uniprot ID: | P97480 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Product Usage Information
IHC/IF | Mouse, Rat | 1: 1000-1: 2000 | hippocampus, striatum, testis |
Background
Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1 (By similarity). Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. The phosphatase activity has been shown in vitro. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity.
Images
| Immunohistochemistry analysis of paraffin embedded mouse hippocampus using EYA3 (GB114369) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded rat striatum using EYA3 (GB114369) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded rat testis using EYA3 (GB114369) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
