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The LiFluor™ 488 EdU Imaging Kit stands out as a superior alternative to the conventional BrdU cell proliferation assay, meticulously optimized for use in fluorescence microscopy. This innovative assay utilizes EdU, a modified thymidine analogue, which is efficiently incorporated into newly synthesized DNA strands. The DNA is then labeled with a vibrant, photostable LiFluor™ dye through a swift and highly-specific click chemistry reaction.
Key Features
• Simple: Labeling is complete in two steps.
• Efficient: No denaturation steps or harsh treatment required.
• Content-rich results: Better preservation of cell morphology, antigen structure, and DNA integrity.
• Consistent: Not dependent on variable antibody lots for detection.
Specifications
1. Platform: Fluorescence Microscope
2. Detection Method: Fluorescent
3. Ex/Em: 495/520 nm
Applications
Cell proliferation analysis in vitro and in vivo.
Components
1. EdU: 2×1 ml
2. LiFluor 488 azide: 100 µl
3. EdU reaction buffer: 50 ml
4. CuSO4: 1 ml
5. EdU buffer additive： 200 mg
6. Hoechst 33342: 70 µl
Storage
Store at -20°C and protect from light.
