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| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | FITC rabbit polyclonal |
| Synonyms: | 5-Isothiocyanat Fluorescein, FITC, Fluorescein |
| Immunogen: | Synthesized peptide derived from FITC |
| Specificity: | This antibody detects FITC conjunted protein or antibody. |
| Source: | Rabbit |
| Concentration: | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit ascites by affinity-chromatography using specific immunogen. |
Product Usage Information
| Applications | Dilution |
| Elisa | 1: 5000-1：10000 |
Background
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. This rabbit antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody. Moreover, this rabbit antibody used with HRP goat anti-rabbit secondary antibody, converts fluorescent signal to DAB dyeing.
Images
| ELISA analysis of FITC (GB111630) at dilution of 1: 5000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
