Fluo-3, AM is a membrane permeant probe and can be passively loaded in cells by simple incubation. Once inside the cell, Fluo-3 becomes fluorescent in the presence of free Ca2+. Fluo-3 AM is widely used in flow cytometry, confocal laser scanning microscopy and fluorescence spectroscopy. Fluo-3, AM has proved particularly valuable for determining GPCR function via cell-based high-throughput screening.
Specifications
1. Ex/Em: 488/525 nm
2. Detection Method: Fluorescent
3. Molecular Formula: C51H50Cl2N2O23
4. Molecular Weight: 1129.85
5. CAS Number: 121714-22-5
Applications
Calcium indicator
Component
1. Fluo-3, AM: 1 mg
Storage
Store at -20°C and protect from light.