Your shopping cart is empty!
| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Glutamate decarboxylase 2 |
Synonyms: | GAD2, GAD65, glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, DCE 2, Glutamic Acid Decarboxylase 2; Glutamic Acid Decarboxylase 65 |
Immunogen: | Recombinant protein corresponding to Mouse GAD65 |
Uniprot ID: | P48320 |
Isotype: | IgG2a,κ |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cytoplasm |
Predicted MW. / Observed MW. | 65 kDa / 65 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1:500-1:1000 | brain, cerebral cortex, hippocampus |
IHC/IF | Mouse, Rat | 1:500-1:2000 | brain, cerebellum, epencephalon |
Background
Glutamate decarboxylase or glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD uses PLP as a cofactor. In mammals, GAD exists in two isoforms with molecular weights of 67 and 65 kDa (GAD67 and GAD65). GAD67 and GAD65 are expressed in the brain where GABA is used as a neurotransmitter, and they are also expressed in the insulin-producing β-cells of the pancreas. GAD65, synthesizes GABA for neurotransmission, and therefore is only necessary at nerve terminals and synapses. In order to aid in neurotransmission, GAD65 forms a complex with Heat Shock Cognate 70 (HSC70), cysteine string protein (CSP) and Vesicular GABA transporter VGAT, which, as a complex, helps package GABA into vesicles for release during neurotransmission.
Images
|
Western blot analysis of GAD65 (GB12562) at dilution of 1: 1000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse cerebellum using GAD65 (GB12562) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded rat brain using GAD65 (GB12562) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using GAD65 (GB12562) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat brain using GAD65 (GB12562) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse epencephalon using GAD65 (GB12562) at dilution of 1: 1000 |
|
Immunofluorescent analysis of paraformaldehyde-fixed rat epencephalon using GAD65 (GB12562) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
