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| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Mouse |
| Isotype: | IgG2b,Kappa |
| Conjugation: | Unconjugated |
| Gene Name: | glyceraldehyde-3-phosphate dehydrogenase |
| Gene Symbol: | GAPDH |
| Synonyms: | G3PD; GAPD; HEL-S-162eP; GAPDH |
| Gene ID: | 2597 |
| UniProt ID: | P04406 |
| Clone ID: | 7H8B3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (P04406). |
| Dilution: | WB 1:5000-1:50000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.60 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of various lysates using GAPDH Mouse mAb (AC002) at 1:10000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of lysates from HeLa cells using GAPDH Mouse mAb (AC002) at 1:5000-1:1280000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) Exposure time: 20 s. |
 | Immunohistochemistry analysis of paraffin-embedded Human kidney using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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