Reactivity: | H,M,R |
Applications: | IHC/IF |
Host Species: | Mouse |
Clonality: | Monoclonal |
Gene Name: | Glucagon-like peptide 1 |
Synonyms: | GCG, GLP1, GLP2, GRPP, glucagon, Glucagon, GLP-1, GLP, GLP-2, GRPP Oxyntomodulin, OXM, Proglucagon, OXY |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human GLP1 |
Uniprot ID: | P01275 |
Isotype: | IgG2b,k |
Purity: | Affinity purification |
Subcellular location: | Secreted |
Predicted MW. / Observed MW. | 3.3-18 kDa / 3.3-18 kDa |
Product Usage Information
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1500 | pancreas, Small intestine |
Background
Glucagon Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
Images
IHC analysis of GLP1(GB123493). Sample: Human pancreas, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. | |
IHC analysis of GLP1(GB123493). Sample: Human pancreas, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. | |
IHC analysis of GLP1(GB123493). Sample: Human Small intestine, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. | |
IHC analysis of GLP1(GB123493). Sample: Mouse pancreas, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. | |
IHC analysis of GLP1(GB123493). Sample: Mouse Small intestine, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. | |
IHC analysis of GLP1(GB123493). Sample: Mouse Small intestine, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. | |
IHC analysis of GLP1(GB123493). Sample: Rat pancreas, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |