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-23%
Anti-Glutathione Peroxidase 4 Mouse mAb (100 μl)
| Reactivity: | H,M,R & M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Phospholipid hydroperoxide glutathione peroxidase |
Synonyms: | PHGPx, Glutathione peroxidase 4, GPx-4, GSHPx-4, Gpx4, snGPx, snPHGPx |
Immunogen: | Recombinant protein corresponding to Mouse GPX4 |
Uniprot ID: | O70325 |
Isotype: | IgG2a,κ |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 22 kDa / 22 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | testis, brain |
IHC/IF | Mouse, Rat | 1: 500-1: 1000 | testis |
ICC/IF | Human, Mouse, Rat | 1:100-1:400 | HeLa |
Background
GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers . It presents primarily in testis.
Images
| Western blot analysis of GPX4 (GB124327) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded rat testis using GPX4 (GB124327) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse testis using GPX4 (GB124327) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using GPX4(GB124327) at dilution of 1:100-1:400 |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
