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Recombinant Anti-GST Tag Rabbit mAb (100 μl)
| Reactivity: | |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Gene Name: | GST Tag |
Synonyms: | Glutathione S-Transferase, GST, gst tag |
Immunogen: | Recombinant GST protein |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 26 kDa / 30 kDa |
Product Usage Information
WB | Species independent | 1: 1000-1: 3000 | Transfected HEK-293F cells |
Background
GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.
Images
| WB analysis of GST Tag (GB15085). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1:3000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG(GB23303), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
