GreenFect™ Transfection Reagent (0.75 ml)

GreenFect™ Transfection Reagent is a lipid-based transfection reagent that forms a complex with DNA or RNA, and transports the complex into a variety of adherent and suspension cell lines. GreenFect™ Transfection Reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells. This reagent has been tested to work the same efficiency as Lipofectamine 3000 Reagent, and used for the transfection of both DNA and RNA into eukaryotic cells even in the presence of serum.
 

Key Features

• Superior transfection efficiency for difficult-to-transfect cells.

• Does not require removal of serum or culture medium.

• Does not require washing or changing of medium after transfection.

• Improved cell viability: Gentle on your cells with low toxicity.

• Versatile: One reagent for DNA, RNA, and co-transfection.
 

Applications

• Hard-to-transfect cell transfection
 

Components

1. GreenFect™ Reagent: 0.75 ml
2. Enhancer Reagent: 0.75 ml
 

Storage

Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Case Study

Assessment of GreenFect™ transfection efficiencies using GFP visualization. A549, NIH3T3, HepG2, and MCF7 cell lines were transfected with a GFP-expressing plasmid using the indicated transfection reagents. Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter).