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Recombinant Anti-HA Tag Mouse mAb (100 μl)
| Reactivity: | |
| Applications: | WB & ICC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | HA Tag |
Synonyms: | HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain |
Immunogen: | KLH conjugated Synthetic peptide corresponding to YPYDVPDYA |
Isotype: | IgG |
Purity: | Affinity purification |
Product Usage Information
WB | Tag (All species) | 1: 1000-1: 2000 | HA-tag fusion protein |
ICC/IF | Tag (All species) | 1: 1000-1: 2000 | transfected CHO cells |
Special note
Epitope labeling has been proved to be an effective method that can be performed on target proteins. Tag antibody can be used in a variety of applications, including ChIP, CoIP, ELISA, FC, IF, IHC, IP, WB and so on.
Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.
Images
| Western blot analysis of HA Tag (GB151252) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde fixed transfected CHO cells using HA Tag (GB151252) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
