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Anti-HECTD1 Rabbit pAb (100 μl)
| Reactivity: | R & R |
| Applications: | IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | HECTD1 rabbit polyclonal |
Gene Name: | E3 ubiquitin-protein ligase HECTD1 |
Synonyms: | HECT domain-containing protein 1, HECT-type E3 ubiquitin transferase HECTD1, Protein open mind, Hectd1, Kiaa1131, Opm, HECTD1 |
Immunogen: | Recombinant protein corresponding to Mouse HECTD1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Rat | 1: 1000-1: 2000 | brain |
IF | Rat | 1: 1000-1: 2000 | skeletal muscle, brain |
Background
HECTD1 (E3 ubiquitin-protein ligase, which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates . Mediates 'Lys-63'-linked polyubiquitination of HSP90AA1 which leads to its intracellular localization and reduced secretion . Negatively regulating HSP90AA1 secretion in cranial mesenchyme cells impairs their emigration and is consequently essential for the correct development of the cranial neural folds and neural tube closure.It is also involved in sporadic Parkinson’s disease.
Images
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Immunohistochemistry analysis of paraffin-embedded rat brain using HECTD1 (GB111394) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat skeletal muscle using HECTD1 (GB111394) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat brain using HECTD1 (GB111394) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
