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Anti-HLA Class II DRB1 Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | HLA class II histocompatibility antigen, DRB1 beta chain |
Synonyms: | DR4, DRB1, DW2.2/DR2.2, HLA DR1B, HLA DRB, HLA DRB1, HLA DRB1*, HLA DRB2, HLA-DRB1, MHC class II antigen DRB1*15, SS1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human HLA Class II DRB1 |
Uniprot ID: | P01911 |
Isotype: | lgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Product Usage Information
IHC/IF | Human | 1: 300-1: 600 | ovarian cancer, lung cancer, liver cancer, lung, liver, colon |
Background
A beta chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the alpha chain HLA-DRA, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DRB1-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins
Images
| IHC analysis of HAL (GB115523). Sample: Human ovarian cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of HAL (GB115523). Sample: Human lung cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of HAL (GB115523). Sample: Human liver cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of HAL (GB115523). Sample: Human lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of HAL (GB115523). Sample: Human liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of HAL (GB115523). Sample: Human colon (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
