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| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | HMGN1 rabbit polyclonal antibody |
Gene Name: | Non-histone chromosomal protein HMG-14 |
Synonyms: | MGC104230, MGC117425, FLJ31471, Hmg14, High mobility group nucleosome-binding domain-containing protein 1, High mobility group protein 14, High-mobility group nucleosome binding domain 1, FLJ27265 |
Immunogen: | Recombinant protein corresponding to Mouse HMGN1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. | 10 kDa |
Observed MW. | 17 kDa |
Uniprot ID: | P18608 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | liver, spleen, lung |
IHC/IF | Mouse, Rat | 1: 500-1: 2000 | lung, skin, testis |
Background
HMGN1 (high-mobility group nucleosome binding protein 1) is a member of the HMG super family of nonhistone chromatin-binding proteins, which which regulate chromatin structure and gene transcription. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. It may participate in the process which maintains transcribable genes in an unique chromatin conformation.
Images
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Western blot analysis of hmgn1 (GB11978) at dilution of 1: 500 |
 | Immunohistochemistry of paraffin embedded mouse lung using hmgn1 (GB11978) at dilution of 1: 1800 (250x lens) |
 | Immunohistochemistry of paraffin embedded mouse skin using hmgn1 (GB11978) at dilution of 1: 1800 (200x lens) |
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Immunohistochemistry of paraffin embedded rat lung using hmgn1 (GB11978) at dilution of 1: 1800 (250x lens) |
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Immunohistochemistry of paraffin embedded rat skin using hmgn1 (GB11978) at dilution of 1: 1800 (200x lens) |
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Immunohistochemistry of paraffin embedded rat testis using hmgn1 (GB11978) at dilution of 1: 1800 (250x lens) |
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Immunofluorescence of paraffin embedded mouse lung using hmgn1 (GB11978) at dilution of 1: 1000 (250x lens) |
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Immunofluorescence of paraffin embedded mouse testis using hmgn1 (GB11978) at dilution of 1: 1000 (350x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
