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| Reactivity: | |
| Applications: | WB |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | horseradish peroxidase |
Immunogen: | Recombinant protein corresponding to HRP |
Isotype: | IgG2b,k |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 44 kDa / 44 kDa |
Product Usage Information
WB | Tag (All species) | 1: 500-1: 1000 | HRP Recombinant protein |
Background
HRP (horseradish peroxidase) is an enzyme frequently used as an indicator in biochemical assays. It is used to amplify signals and enhance a target’s detectability. HRP acts on various chromogenic substrates to produce many different colors and acts on chemiluminescent substrates to produce light. HRP is often conjugated to an antibody to detect a target molecule. The antibody specifically locates the target, and HRP, in company with a substrate, and produces a detectable signal. Anti-HRP antibodies are designed to reliably detect HRP in various applications.
Images
| Western blot analysis of HRP (GB122199) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
