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Applications: | ELISA |
Host Species: | Rabbit |
Isotype: | IgD |
Conjugation: | HRP |
Immunogen: | Monkey IgD |
Dilution: | ELISA 1:5000 -1:10000 |
Purification Method: | Affinity purification |
Buffer: | PBS with 0.05% proclin300,0.05% BSA,50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual |
Images
![]() | Dose response curve of HRP conjugated Monkey IgD mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Monkey IgD mAb in a 3 fold serial dilution from 1μg/mL to 6.97×10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Monkey IgD mAb has highly specific recognition of Monkey IgD while no or minimal cross reactivity to Monkey IgA、Monkey IgE、Monkey IgG3、Human IgD、Mouse IgD. |