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Anti-Heme-regulated inhibitor Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M,R & M,R |
| Applications: | WB & IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Heme-regulated inhibitor rabbit polyclonal |
Gene Name: | Eukaryotic translation initiation factor 2-alpha kinase 1 |
Synonyms: | Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase, Eif2ak1, Heme-controlled repressor, HCR, Heme-regulated inhibitor, Hri |
Immunogen: | Recombinant protein corresponding to Mouse Heme-regulated inhibitor |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 70 kDa |
Observed MW. | 70 kDa |
Subcellular location: | |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | HEK293 |
IHC | Mouse, Rat | 1: 800-1: 1600 | bone, lung, inflammation lung |
IF | Mouse, Rat | 1: 500-1: 1000 | bone, lung, inflammation lung |
Background
The Eif2ak1 Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells, controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties.
Images
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Western blot analysis of Eif2ak1 (GB111094) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded mouse bone using Eif2ak1 (GB111094) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded rat lung using Eif2ak1 (GB111094) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded mouse inflammation lung using Eif2ak1 (GB111094) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
