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Anti-Histone H2A.X Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Histone H2A.X rabbit polyclonal |
Gene Name: | Histone H2AX |
Synonyms: | H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Histone H2A.X |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 15 kDa |
Observed MW. | 15 kDa |
Subcellular location: | |
Uniprot ID: | P16104, P27661 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver, kidney, colon |
IHC | Human, Mouse, Rat | 1: 1000-1: 2000 | colon cancer, colon, kidney, testis |
IF | Human, Mouse, Rat | 1: 500-1: 1000 | colon cancer, colon, kidney, testis |
Background
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Images
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Western blot analysis of H2AX (GB111508) at dilution of 1: 1000 Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Mouse colon tissue lysate Lane 7: Rat liver tissue lysate Lane 8: Rat kidney tissue lysate |
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Immunohistochemistry analysis of paraffin-embedded human colon cancer using H2AX (GB111508) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded mouse colon using H2AX (GB111508) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat colon using H2AX (GB111508) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded mouse kidney using H2AX (GB111508) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat kidney using H2AX (GB111508) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded mouse testis using H2AX (GB111508) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat testis using H2AX (GB111508) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed human colon cancer using H2AX (GB111508) at dilution of 1: 600 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat colon using H2AX (GB111508) at dilution of 1: 600 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse kidney using H2AX (GB111508) at dilution of 1: 600 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using H2AX (GB111508) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
