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Anti-Histone H3.3 Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Histone H3.3 rabbit polyclonal antibody |
Gene Name: | Histone H3.3 |
Synonyms: | H3F3A, H3.3A, H3F3, H3 histone, family 3A, H3 histone family member 3A |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Histone H3.3 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 15 kDa |
Observed MW. | 17 kDa |
Uniprot ID: | P84243, P84244, P84245 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1:500-1:1000 | lung, brain |
Background
Histone H3.3 is a protein that in humans is encoded by the H3F3A gene.Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Images
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Western blot analysis of Histone H3.3 (GB11026) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
