Reactivity: | H,M,R & H,M,R & H,M,R |
Applications: | WB & IHC/IF & ICC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Histone H4 |
Synonyms: | H4C1, H4/A, H4C2, H4C3, H4C4, H4/I, HIST1H4BAND, HIST1H4CAND, HIST1H4E |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Histone H4 |
Uniprot ID: | P62805 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 11 kDa / 11 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver, lung, testis |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | tonsil, lung, liver |
ICC/IF | Human, Mouse, Rat | 1: 100-1: 500 | HeLa, NIH/3T3 |
Background
Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. remodeling.
Images
Western blot analysis of Histone H4 (GB111688) at dilution of 1: 500 | |
Immunohistochemistry analysis of paraffin embedded human stomach using Histone H4 (GB111688) at dilution of 1: 1000 | |
Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using Histone H4 (GB111688) at dilution of 1: 500 | |
Immunohistochemistry analysis of paraffin-embedded human tonsil using Histone H4 (GB111688) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin-embedded human lung using Histone H4 (GB111688) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin-embedded human liver using Histone H4 (GB111688) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin-embedded human colon using Histone H4 (GB111688) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin-embedded rat colon using Histone H4 (GB111688) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin-embedded mouse colon using Histone H4 (GB111688) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin-embedded human ovary using Histone H4 (GB111688) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin-embedded human esophagus using Histone H4 (GB111688) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin-embedded rat kidney using Histone H4 (GB111688) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin-embedded rat kidney using Histone H4 (GB111688) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin-embedded rat testis using Histone H4 (GB111688) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin-embedded mouse testis using Histone H4 (GB111688) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin-embedded mouse small intestine using Histone H4 (GB111688) at dilution of 1: 1000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |