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Anti-Histone H4 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Histone H4 rabbit polyclonal |
Gene Name: | Histone H4 |
Synonyms: | H4c1, Hist1h4a, H4c2, H4-53, Hist1h4b, H4c3, H4-12, Hist1h4c, H4c4, Hist1h4d, Hist1h4f, H4c6, H4c14, Hist2h4, Hist2h4a |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Histone H4 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 11 kDa |
Observed MW. | 11 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | colon, testis |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | lung cancer, colon, ovarian cancer, lung, testis, skin, kidney, small intestine, heart, cholangiocarcinoma, cervical cancer |
ICC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | Hela, NIH3T3 |
Background
Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Images
| Western blot analysis of Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human lung cancer using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded rat colon using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse colon using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human ovarian cancer using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse lung using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded rat lung using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse testis using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded rat testis using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse skin using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse kidney using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded rat kidney using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse small intestine using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded rat small intestine using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse heartusing Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded rat heart using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human cholangiocarcinoma using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human cervical cancer using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human small intestine using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat kidney using Histone H4 (GB111842) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed Hela cell using Histone H4 (GB111841) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH373 cell using Histone H4 (GB111841) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
