Reactivity: | H & H |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | Hsp27 rabbit polyclonal antibody |
Gene Name: | Heat shock protein beta-1 |
Synonyms: | HspB1, Estrogen-regulated 24 kDa protein, 28 kDa heat shock protein, Heat shock 27 kDa protein, HSP27, Stress-responsive protein 27, SRP27, HSP28 |
Immunogen: | Recombinant protein corresponding to Human Hsp27 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. | 23 kDa |
Observed MW. | 27 kDa |
Uniprot ID: | P04792 |
Images
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 10000-1: 20000 | HeLa, MCF7, A549, SH-SY5Y, SKOV3 |
IHC/IF | Human | 1: 1000-1: 3000 | breast cancer, lung cancer |
Background
HSPB1, also known as heat shock protein 27 (Hsp27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma.
Images
Western blot analysis of HSP27 (GB111176) at dilution of 1: 10000 |
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Immunohistochemistry of paraffin embedded human breast cancer using HSP27 (GB111176) at dilution of 1: 1500 (400x lens) |
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Immunohistochemistry of paraffin embedded human lung cancer using HSP27 (GB111176) at dilution of 1: 1500 (400x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |