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Anti-Hsp60 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | HSP60 rabbit polyclonal antibody |
Gene Name: | 60 kDa heat shock protein, mitochondrial |
Synonyms: | HSP60, HSP-60, HuCHA60, CPN60, 60 KDa Chaperonin, P60 Lymphocyte Protein, Heat Shock Protein 60 |
Immunogen: | Recombinant protein corresponding to Mouse HSP60 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. | 60 kDa |
Observed MW. | 60 kDa |
Uniprot ID: | P10809, P63039, P63038 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 250-1: 500 | liver, lung |
IHC/IF | Human, Mouse, Rat | 1: 300-1: 1500 | kidney, liver, lung, lung cancer, placenta, renal cancer |
Background
Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. Through the extensive study of groEL, HSP60’s bacterial homolog, HSP60 has been deemed essential in the synthesis and transportation of essential mitochondrial proteins from the cell's cytoplasm into the mitochondrial matrix.
Images
| Western blot analysis of HSP60 (GB112464) at dilution of 1: 250 |
| Immunohistochemistry of paraffin embedded human kidney using HSP60 (GB112464) at dilution of 1: 1000 (400x lens) |
| Immunohistochemistry of paraffin embedded human lung using HSP60 (GB112464) at dilution of 1: 1000 (400x lens) |
| Immunohistochemistry of paraffin embedded human placenta using HSP60 (GB112464) at dilution of 1: 350 (400x lens) |
| Immunohistochemistry of paraffin embedded human renal cancer using HSP60 (GB112464) at dilution of 1: 1000 (400x lens) |
| Immunofluorescence of paraffin embedded human kidney using HSP60 (GB112464) at dilution of 1: 400 (400x lens) |
 | Immunofluorescence of paraffin embedded human liver using HSP60 (GB112464) at dilution of 1: 400 (400x lens) |
 | Immunofluorescence of paraffin embedded human lung cancer using HSP60 (GB112464) at dilution of 1: 400 (400x lens) |
 | Immunofluorescence of paraffin embedded human placenta using HSP60 (GB112464) at dilution of 1: 400 (400x lens) |
 | Immunofluorescence of paraffin embedded mouse kidney using HSP60 (GB112464) at dilution of 1: 400 (400x lens) |
 | Immunofluorescence of paraffin embedded mouse liver using HSP60 (GB112464) at dilution of 1: 400 (400x lens) |
| Immunofluorescence of paraffin embedded rat kidney using HSP60 (GB112464) at dilution of 1: 400 (400x lens) |
| Immunofluorescence of paraffin embedded rat liver using HSP60 (GB112464) at dilution of 1: 400 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
