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Recombinant Anti-Hsp60 antibody (HRP Conjugated) (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | |
| Clonality: | |
| Gene Name: | 60 kDa heat shock protein, mitochondrial |
Synonyms: | HSP60, HSP-60, HuCHA60, CPN60, 60 KDa Chaperonin, P60 Lymphocyte Protein, Heat Shock Protein 60 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse HSP60 |
Uniprot ID: | P63038 |
Isotype: | IgG1 |
Conjugation | HRP |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 60 kDa / 60 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | HeLa, Hep G2 |
Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-Hsp60 antibody(ZB15243-HRP) for 3-4 hours at room temperature (20-25°C) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. Through the extensive study of groEL, HSP60’s bacterial homolog, HSP60 has been deemed essential in the synthesis and transportation of essential mitochondrial proteins from the cell's cytoplasm into the mitochondrial matrix. Further studies have linked HSP60 to diabetes, stress response, cancer and certain types of immunological disorders.
Images
| WB analysis of Hsp60(ZB15243-HRP). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-Hsp60 antibody 1: 1000, 4°C overnight. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
