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| Reactivity: | M,R & M,R |
| Applications: | IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | IOP2 rabbit polyclonal |
Gene Name: | Nuclear prelamin A recognition factor |
Synonyms: | Narf, NARF, IOP2, Nuclear prelamin A recognition factor, Iron only hydrogenase like protein 2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse IOP2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Mouse, Rat | 1: 2000-1: 3000 | lung, pancreas |
IF | Mouse, Rat | 1: 1000-1: 1500 | lung, pancreas |
Background
Renylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein–protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The encoded protein binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases.
Images
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Immunohistochemistry analysis of paraffin-embedded mouse lung using IOP2 (GB11748) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat lung using IOP2 (GB11748) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat pancreas using IOP2 (GB11748) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
