Your shopping cart is empty!
| Reactivity: | H,M & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | IRS1 rabbit polyclonal antibody |
Gene Name: | Insulin receptor substrate 1 |
Synonyms: | HIRS 1, HIRS1, IRS 1, Insulin receptor substrate 1, IRS-1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse IRS1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cell menbrane, Nucleus |
Predicted MW. | 132 kDa |
Observed MW. | 180 kDa |
Uniprot ID: | P35568, P35569, P35570 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse | 1: 500-1: 1000 | skeletal muscle |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 2000 | brain, breast cancer, colon, liver, Skeletal muscle, testis |
Background
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Images
 |
Western blot analysis of IRS-1 (GB111506) at dilution of 1: 500 |
 |
Immunohistochemistry of paraffin embedded human breast cancer using IRS-1 (GB111506) at dilution of 1: 1100 (400x lens) |
 |
Immunohistochemistry of paraffin embedded mouse brain using IRS-1 (GB111506) at dilution of 1: 1100 (400x lens) |
 |
Immunohistochemistry of paraffin embedded mouse brain using IRS-1 (GB111506) at dilution of 1: 1100 (400x lens) |
 |
Immunohistochemistry of paraffin embedded rat colon using IRS-1 (GB111506) at dilution of 1: 1100 (400x lens) |
 |
Immunohistochemistry of paraffin embedded rat skeletal muscle using IRS-1 (GB111506) at dilution of 1: 1100 (400x lens) |
 |
Immunofluorescence of paraffin embedded human breast cancer using IRS-1 (GB111506) at dilution of 1: 600 (400x lens) |
 |
Immunofluorescence of paraffin embedded mouse liver using IRS-1 (GB111506) at dilution of 1: 600 (400x lens) |
 |
Immunofluorescence of paraffin embedded mouse testis using IRS-1 (GB111506) at dilution of 1: 600 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
