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| Reactivity: | H,M,R & H |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Allograft inflammatory factor 1 |
Synonyms: | AIF-1, IBA, IRT-1, IRT1, allograft, AIF-1, AIF1, G1, IBA1, IRT 1, Protein G1, Ionized calcium-binding adapter molecule 1, MRF1, Microglia response factor, BART 1, AIF1 protein |
Immunogen: | Recombinant protein corresponding to Human Iba1 |
Uniprot ID: | P55008 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cell membrane |
Predicted MW. / Observed MW. | 10,14,17 kDa / 14-17 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 300-1: 600 | brain, testis |
IHC/IF | Human | 1: 200-1: 400 | colon, tonsil |
Background
AIF1, also named as G1, IBA1 or daintain/AIF-1, is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Despite a lack of detailed knowledge on the in vivo physiological functions of AIF-1, there is growing evidence that shows its aberrant expression contributes to the pathogenesis of many autoimmune diseases, including rheumatoid arthritis. AIF1 is an actin-binding protein that enhances membrane ruffling and RAC activation. It enhances lymphocyte migration and the actin-bundling activity of LCP1.
Images
| Western blot analysis of IBA-1 (GB114490) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded human colon using IBA-1 (GB114490) at dilution of 1: 200 |
| Immunohistochemistry analysis of paraffin embedded human tonsil using IBA-1 (GB114490) at dilution of 1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
