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LiFluor™ Fluorescent Dyes
Fluorescent labeling of biomolecules enables researchers to detect specific components of complex biological assemblies with exquisite sensitivity in applications ranging from imaging and flow cytometry, to Western blots. LifeSct offers a full spectrum of LiFluor™ Dyes for labeling antibodies, proteins, peptides, ligands, synthetic oligonucleotides and other biomolecules for use with immunochemistry, fluorescence in situ hybridization (FISH), cell tracing, receptor labeling, and cytochemistry applications as well as probing biological structure, function and interactions. LifeSct offers a series of superior bright, highly photo-stable and excellent water-soluble LiFluor™ dyes spanning the visible and near-infrared (IR) spectrum for labeling biomolecules, especially proteins and nucleic acids.
Key Features
• LiFluor™ fluorescent dyes have superior brightness and good photostability
• The dyes are insensitive to pH change and has good water solubility
• Multiplexing: across spectrum from UV to near-IR
| Fluorescent Dye | Ex/Em (nm) | Laser Type | Advantages | Replacement |
| LiFluor™ 350 Blue | 350/440 | UV | Highly fluorescent; Highly water- soluble and pH insensitive | AMCA, Alexa Fluor 350, DyLight 350 |
| LiFluor™ 405 Blue | 405/450 | 405 nm laser | Brighter than Pacific Blue; Less spill-over in the green channel | Pacific Blue, BD Horizon V450 |
| LiFluor™ 405 Green | 412/500 | 405 nm laser | Highly fluorescent; Less spill- over in the green channel | BD Horizon V500 |
| LiFluor™ 488 Green | 495/520 | 488 nm laser | Extremely photostable; Highly fluorescent and water-soluble | Alexa Fluor 488, DyLight 488, Cy2, FITC |
| LiFluor™ 546 Red | 556/575 | 532, 543, 546 or 555 nm laser | Extremely photostable; Highly fluorescent and water-soluble | Alexa Fluor 546, Cy3, TAMRA |
| LiFluor™ 555 Red | 553/565 | 532, 543, 546 or 555 nm laser | Brighter than TAMRA; Highly photostable and water-soluble | Alexa Fluor 555, DyLight 549, Cy3, TAMRA |
| LiFluor™ 568 Red | 578/602 | 543, 546, 555 or 568 nm laser | Highly water-soluble and pH insensitive; Highly fluorescent | Alexa Fluor 568, ATTO 565, Rhodamine Red |
| LiFluor™ 594 Red | 590/615 | 555 or 568 nm laser | Brighter than Texas Red; Highly water-soluble and pH insensitive | Alexa Fluor 594, DyLight 594, Texas Red |
| LiFluor™ 610 Red | 610/625 | 555 or 568 nm laser | Highly fluorescent; Highly water- soluble and pH insensitive | Alexa Fluor 610 |
| LiFluor™ 635 Red | 630/650 | 633 or 635 nm laser | Highly fluorescent; Highly water- soluble and pH insensitive | Alexa Fluor 635, DyLight 633 |
| LiFluor™ 647 Red | 650/666 | 633 or 635 nm laser | Brighter than Cy5; Highly water- soluble and pH insensitive | Alexa Fluor 647, DyLight 649, Cy5 |
| LiFluor™ 680 Red | 680/700 | 680 or 685 nm laser | Brighter than Cy5.5; Highly water-soluble and pH insensitive | Alexa Fluor 680, DyLight 680, Cy5.5 |
| LiFluor™ 750 Red | 755/770 | 680 or 685 nm laser | Highly fluorescent; Highly water- soluble and pH insensitive | Alexa Fluor 750, DyLight 750, Cy7 |
Case Study
Comparison of relative fluorescence of goat anti-rabbit IgG antibody conjugates of LiFluor™ dyes and Cy dyes at different dye:protein ratios in the conjugate.
Comparison of the photobleaching rates of LiFluor™ 488 goat anti-mouse IgG antibody conjugate (top) with FITC goat anti-mouse IgG antibody conjugate (bottom).