SuperECL Western Blotting Detection Kits

SuperECL Western Blotting Detection Kits

LifeSct offers two types of super sensitive chemiluminescent substrates for Western blot detection with horseradish peroxidase enzyme (HRP). The signal from ECL substrates can be captured and documented using X-ray film or a capable CCD camera imager.

SensitivityPrimary Ab DilutionSecondary Ab Dilution
SuperECL™Picogram to low femtogram1:1,000-1:30,0001:50,000-1:200,000
SuperECL™ PlusHigh Femtogram1:5,000-1:30,0001:100,000-1:300,000

Key Features

• High sensitivity: detect low-picogram to high-femtogram amounts of target protein on nitrocellulose or PVDF membrane.
• High signal stability: incubated blots provide stable signal duration over 4 hour time period.
• Stable reagent: reagent is stable for one year.
• Cost-effective: use much less antibody.

Product Name
Cat. #
PriceView
SuperECL™ Western Blotting Detection Kit (2×50 ml)M0013-05$149
SuperECL™ Western Blotting Detection Kit (2×250 ml)M0013-25$349
SuperECL™ Plus Western Blotting Detection Kit (2×50 ml)M0053-05$249
SuperECL™ Plus Western Blotting Detection Kit (2×100 ml)M0053-10$389

Case Study

ECL Western Blotting Detection Kit

The comparison of SuperECL™ plus and SuperSignal™ (Thermo) on exposure time.


ECL WB Detection Kit

The comparison on exposure time(Left); The comparison on signal duration (Right).


ECL Detection Kit
The comparison of sensitivity of SuperECL™, SuperECL™ Plus, and Immobilon™ (Millipore) Western blot substrate (Left); The comparison on antibody dilution (Right).

Customer Testimonials

"We tested the SuperECL Western Blotting Detection Kit sample, and it did work well, same as the Pierce’s one. BTW, your LiPure™ Plasmid Mini Kit works very well, better than others we used. Also I have been trying to convince our purchasing department you are a good supplier to be considered to add to our vendor list."

------ University of Pittsburgh, Dr. Qingde Wang, PI

"I have been using LifeSct’s SuperECL Western Blotting Detection Kit for a while and recently also start using LifeSct’s SuperECL Plus Western Blotting Detection Kit product. Both products work perfectly and the results are even better than pico and femto level products from the major brand I used before. I am very happy with these wonderful products."

------ NIH/NHLBI, Dr. Pingyuan Wang, PHD

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