KD-Validated CDX2 Rabbit mAb (20 μl)

KD-Validated CDX2 Rabbit mAb (20 μl)

Brand: Abclonal
Cat. #: A20222
Availability: In Stock
$148.00
-+
Reactivity: Human    
Applications: WB, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Monoclonal antibody    
Gene Name: Caudal type homeobox 2
Gene Symbol: CDX2
Synonyms: CDX3; CDX-3; CDX2/AS; X2
Gene ID: 1045
UniProt ID: Q99626
Clone ID: 4Y1O3
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CDX2 (Q99626 ).
Dilution: WB 1:500-1:1000
Purification Method: Affinity purification
Concentration: 0.50 mg/mL
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-CDX2 monoclonal antibody




Background

The gene CDX2 is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis.




Images

CDX2 rabbit mAb Western blot analysis of lysates from SW620 cells, using [KD Validated] CDX2 Rabbit mAb (A20222) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
CDX2 monoclonal antibody Western blot analysis of lysates from wild type(WT) and CDX2 knockdown(KD) 293T cells, using [KD Validated] CDX2 Rabbit mAb (A20222) at1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Caudal type homeobox 2 Western blot analysis of lysates from Mouse colon, using [KD Validated] CDX2 Rabbit mAb (A20222) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.



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