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KD-Validated Casein Kinase 2 alpha (CSNK2A1) Rabbit mAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | casein kinase 2 alpha 1 |
| Gene Symbol: | CSNK2A1 |
| Synonyms: | CKII; Cka1; Cka2; CK2A1; OCNDS; [KD Validated] Casein Kinase 2 alpha (CSNK2A1) |
| Gene ID: | 1457 |
| UniProt ID: | P68400 |
| Clone ID: | 2P3M7 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Casein Kinase 2 alpha (CSNK2A1) (P68400). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.8 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CSNK2A1 monoclonal antibody |
Background
Casein kinase II (CSNK2A1) is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene.
Images
 | Western blot analysis of various lysates, using [KD Validated] Casein Kinase 2 alpha (CSNK2A1) Rabbit mAb (A19683) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from wild type(WT) and Casein Kinase 2 alpha (CSNK2A1) knockdown (KD) 293T cells, using [KD Validated] Casein Kinase 2 alpha (CSNK2A1) Rabbit mAb (A19683) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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