KD-Validated ISG15 Rabbit mAb (20 μl)

KD-Validated ISG15 Rabbit mAb (20 μl)

Brand: Abclonal
Cat. #: A2416
Availability: In Stock
$148.00
-+
Reactivity: Human    
Applications: WB, IF/ICC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Monoclonal antibody    
Gene Name: ISG15 ubiquitin like modifier
Gene Symbol: ISG15
Synonyms: G1P2; IP17; UCRP; IFI15; IMD38; hUCRP; [KD Validated] ISG15
Gene ID: 9636
UniProt ID: P05161
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human ISG15 (NP_005092.1).
Dilution: WB 1:3000-1:20000; IHC 1:50-1:200; IF/IC 1:50-1:200
Purification Method: Affinity purification
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-ISG15 monoclonal antibody




Background

The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.




Images

ISG15 rabbit mAbWestern blot analysis of lysates from wild type(WT) and ISG15 knockdown (KD) 293T cells, using [KD Validated] ISG15 Rabbit mAb (A2416) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Confocal imaging of U-2 OS cells using [KD Validated] ISG15 Rabbit mAb (A2416,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.



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