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KD-Validated KMT5A/SETD8 Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | lysine methyltransferase 5A |
| Gene Symbol: | KMT5A |
| Synonyms: | SET8; SET07; SETD8; PR-Set7; PR/SET07; [KO/KD Validated] KMT5A/SETD8 |
| Gene ID: | 387893 |
| UniProt ID: | Q9NQR1 |
| Clone ID: | 5M9P5 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KMT5A/SETD8 (Q9NQR1). |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-KMT5A monoclonal antibody |
Background
The protein encoded by this gene is a protein-lysine N-methyltransferase that can monomethylate Lys-20 of histone H4 to effect transcriptional repression of some genes. The encoded protein is required for cell proliferation and plays a role in chromatin condensation.
Images
 | Western blot analysis of various lysates, using [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Western blot analysis of lysates from wild type(WT) and KMT5A/SETD8 knockdown (KD) 293T cells, using [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Enhanced Kit (RM00021). Exposure time: 30s. |
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