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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | polypyrimidine tract binding protein 1 |
| Gene Symbol: | PTBP1 |
| Synonyms: | PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I; P1 |
| Gene ID: | 5725 |
| UniProt ID: | P26599 |
| Clone ID: | 10W8V4 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PTBP1 (NP_114368.1). |
| Dilution: | WB 1:1000-1:5000; IHC 1:200-1:2000; IF/IC 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.15 mg/mL |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-PTBP1 monoclonal antibody |
Background
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Images
 | Western blot analysis of lysates from wild type (WT) and PTBP1 knockdown (KD) HeLa cells using [KD Validated] PTBP1 Rabbit mAb (A25184) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of lysates from Mouse lung using [KD Validated] PTBP1 Rabbit mAb (A25184) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse intestine tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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