KD-Validated CD98/SLC3A2 Rabbit PolymAb® (20 μl)

KD-Validated CD98/SLC3A2 Rabbit PolymAb® (20 μl)

Brand: Abclonal
Cat. #: A19880PM
Availability: In Stock
$148.00
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Reactivity: Human, Mouse    
Applications: WB, IF/ICC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Monoclonal antibody    
Gene Name: solute carrier family 3 member 2
Gene Symbol: SLC3A2
Synonyms: 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC
Gene ID: 6520/17254
UniProt ID: P08195/P10852
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 206-630 of human/mouse SLC3A2/CD98hc (NP_002385.3).
Purification Method: Affinity purification
Concentration: 1.57mg/ml
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-SLC3A2 monoclonal antibody




Background

This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.




Images

SLC3A2 rabbit mAbWestern blot analysis of lysates from Rat kidney using [KD Validated]CD98/SLC3A2 Rabbit PolymAb® (A19880PM) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and CD98/SLC3A2 knockdown (KD) HeLa cells using [KD Validated]CD98/SLC3A2 Rabbit PolymAb® (A19880PM) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Confocal imaging of Hep G2 cells using [KD Validated]CD98/SLC3A2 Rabbit PolymAb® (A19880PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of HeLa cells using [KD Validated]CD98/SLC3A2 Rabbit PolymAb® (A19880PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.



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