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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | TIFA inhibitor |
| Gene Symbol: | TIFAB |
| Gene ID: | 497189 |
| UniProt ID: | Q6ZNK6 |
| Clone ID: | 1R6B8 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 62-161 of human TIFAB(NP_001092691.1) |
| Purification Method: | Affinity purification |
| Dilution: | WB 1:1000-1:5000 |
| Concentration: | 1.08 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-TIFAB monoclonal antibody |
Background
TIFAB binds to TIFA (MIM 609028) and inhibits TIFA mediated NF kappa-B (NFKB1; MIM 164011) activation.
Images
 | Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TIFAB using [KD Validated] TIFAB Rabbit mAb (A24024) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from wild type (WT) and TIFAB knockdown (KD) MV-4-11 cells using [KD Validated] TIFAB Rabbit mAb (A24024) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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