KD-Validated TIFAB Rabbit mAb (20 μl)

KD-Validated TIFAB Rabbit mAb (20 μl)

Brand: Abclonal
Cat. #: A24024
Availability: In Stock
$148.00
-+
Reactivity: Human    
Applications: WB, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Monoclonal antibody    
Gene Name: TIFA inhibitor
Gene Symbol: TIFAB
Gene ID: 497189
UniProt ID: Q6ZNK6
Clone ID: 1R6B8
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 62-161 of human TIFAB(NP_001092691.1)
Purification Method: Affinity purification
Concentration: 1.08 mg/mL
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-TIFAB monoclonal antibody




Background

TIFAB binds to TIFA (MIM 609028) and inhibits TIFA mediated NF kappa-B (NFKB1; MIM 164011) activation.




Images

TIFAB rabbit mAbWestern blot analysis of lysates from wild type (WT) and 293T cells transfected with TIFAB using [KD Validated] TIFAB Rabbit mAb (A24024) at 1:2000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
<b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>
Western blot analysis of lysates from wild type (WT) and TIFAB knockdown (KD) MV-4-11 cells using [KD Validated] TIFAB Rabbit mAb (A24024) at 1:2000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
<b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>



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