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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | TNF receptor associated factor 2 |
| Gene Symbol: | TRAF2 |
| Synonyms: | TRAP; TRAP3; RNF117; MGC:45012; [KD Validated] TRAF2 |
| Gene ID: | 7186 |
| UniProt ID: | Q12933 |
| Clone ID: | 3S2A8 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-350 of human TRAF2 (NP_066961.2). |
| Dilution: | WB 1:25000-1:100000 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-TRAF2 monoclonal antibody |
Background
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.
Images
 | Western blot analysis of various lysates, using [KD Validated] TRAF2 Rabbit mAb (A19129) at 1:50000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of various lysates, using [KD Validated] TRAF2 Rabbit mAb (A19129) at 1:50000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of lysates from wild type(WT) and TRAF2 knockdown (KD) 293T cells, using [KD Validated] TRAF2 Rabbit mAb (A19129) at 1:50000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg TRAF2 antibody (A19129). Western blot was performed from the immunoprecipitate using TRAF2 antibody (A19129) at a dilution of 1:50000. |
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