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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | adenine phosphoribosyltransferase |
| Gene Symbol: | APRT |
| Synonyms: | AMP; APRTD |
| Gene ID: | 353 |
| UniProt ID: | P07741 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APRT (NP_000476.1). |
| Dilution: | WB 1:11000-1:110000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.18 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-APRT monoclonal antibody |
Background
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for the gene APRT.
Images
 | Western blot analysis of lysates from MCF7 cells using [KD Validated] APRT Rabbit mAb (A27254) at 1:11000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 0.5s. |
 | Western blot analysis of various lysates using [KD Validated] APRT Rabbit mAb (A27254) at 1:11000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from wild type (WT) and APRT knockdown (KD) 293T cells using [KD Validated] APRT Rabbit mAb (A27254) at 1:11000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 0.5s. |
 | Confocal imaging of 293T cells using [KD Validated] APRT Rabbit mAb (A27254, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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