KD-Validated KMT5A/SETD8 Rabbit mAb (20 μl)

KD-Validated KMT5A/SETD8 Rabbit mAb (20 μl)

Brand: Abclonal
Cat. #: A4136
Availability: In Stock
$148.00
-+
Reactivity: Human    
Applications: WB, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Monoclonal antibody    
Gene Name: lysine methyltransferase 5A
Gene Symbol: KMT5A
Synonyms: SET8; SET07; SETD8; PR-Set7; PR/SET07; [KO/KD Validated] KMT5A/SETD8
Gene ID: 387893
UniProt ID: Q9NQR1
Clone ID: 5M9P5
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KMT5A/SETD8 (Q9NQR1).
Purification Method: Affinity purification
Concentration: 1 mg/ml
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-KMT5A monoclonal antibody




Background

The protein encoded by this gene is a protein-lysine N-methyltransferase that can monomethylate Lys-20 of histone H4 to effect transcriptional repression of some genes. The encoded protein is required for cell proliferation and plays a role in chromatin condensation.




Images

KMT5A rabbit mAbWestern blot analysis of various lysates, using [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of lysates from wild type(WT) and KMT5A/SETD8 knockdown (KD) 293T cells, using [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Enhanced Kit (RM00021).
Exposure time: 30s.



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