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KD-Validated MRPL27 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | mitochondrial ribosomal protein L27 |
| Gene Symbol: | MRPL27 |
| Synonyms: | L27mt; MRPL27 |
| Gene ID: | 51264 |
| UniProt ID: | Q9P0M9 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 49-148 of human MRPL27(NP_057588.1) . |
| Dilution: | WB 1:1000-1:5000; IHC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 1.54 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-MRPL27 polyclonal antibody |
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.
Images
 | Western blot analysis of lysates from wild type (WT) and MRPL27 knockdown (KD) 293T cells using [KD Validated] MRPL27 Rabbit pAb (A25221) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Human cervical squamous cell carcinoma tissue using [KD Validated] MRPL27 Rabbit pAb (A25221) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] MRPL27 Rabbit pAb (A25221) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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