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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | PHD finger protein 11 |
| Gene Symbol: | PHF11 |
| Synonyms: | APY; BCAP; IGEL; IGER; IGHER; NYREN34; NY-REN-34; [KD Validated] PHF11 |
| Gene ID: | 51131 |
| UniProt ID: | Q9UIL8 |
| Clone ID: | 1J3E4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-321 of human PHF11（NP_001035533.1）. |
| Dilution: | WB 1:1000-1:5000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.77 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-PHF11 monoclonal antibody |
Background
This gene encodes a protein containing a PHD (plant homeodomain) type zinc finger. This gene has been identified in some studies as a candidate gene for asthma. Naturally-occurring readthrough transcription may occur from the upstream SETDB2 (SET domain bifurcated 2) gene to this locus. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from Mouse spleen, using [KD Validated] PHF11 Rabbit mAb (A23994) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from wild type(WT) and PHF11 knockdown (KD) 293T cells, using [KD Validated] PHF11 Rabbit mAb (A23994) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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