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KD-Validated Cyclophilin F Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | peptidylprolyl isomerase F |
| Gene Symbol: | PPIF |
| Synonyms: | CYP3; CypD; CyP-M; Cyp-D |
| Gene ID: | 10105 |
| UniProt ID: | P30405 |
| Clone ID: | 5F2L7 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 108-207of human Cyclophilin F (NP_005720.1). |
| Dilution: | WB 1:20000-1:80000; IHC 1:200-1:800; IF/IC 1:200-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 1.98 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-PPIF monoclonal antibody |
Background
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.
Images
 | Western blot analysis of lysates from HAP1 cells using [KD Validated] Cyclophilin F Rabbit mAb (A25355) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of lysates from wild type (WT) and Cyclophilin F knockdown (KD) 293F cells using [KD Validated] Cyclophilin F Rabbit mAb (A25355) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] Cyclophilin F Rabbit mAb (A25355) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] Cyclophilin F Rabbit mAb (A25355) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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