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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | amyloid beta precursor protein |
| Gene Symbol: | APP |
| Synonyms: | AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTF gamma; alpha-sAPP; PP |
| Gene ID: | 351 |
| UniProt ID: | P05067 |
| Clone ID: | 3U3B4 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 671-770 of human APP (P05067). |
| Dilution: | WB 1:1000-1:6000; IF/IC 1:100-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 0.57 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-APP monoclonal antibody |
Background
The gene APP encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from wild type (WT) and APP knockout (KO) 293T cells, using [KO Validated] APP Rabbit mAb (A17911) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of various lysates using [KO Validated] APP Rabbit mAb (A17911) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of HeLa cells using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Confocal imaging of paraffin-embedded 5XFAD Mouse brain and Mouse brain using [KO Validated] APP Rabbit mAb (A17911, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. |
 | Immunoprecipitation analysis of 300 μg extracts from HeLa cells using 3 μg [KO Validated] APP Rabbit mAb (A17911). Western blot was performed from the immunoprecipitate using [KO Validated] APP Rabbit mAb (A17911) at a dilution of 1:1000. |
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